Plant Tissue Cult. 4(2) : 123-129, 1994

Single and Synergistic Effect of Some Vitamins and Antioxidants in Controlling Early Senescence of in vitro Regenerated Plants of Jute (Corchorus olitorius L.)

A.B.M. Mamun Hossain, Golam Ahmed and M.S. Islam

Division of Genetics, Institute of Food and Radiation Biology, Atomic Energy Recearch Establishment, GPO Box No. 3787, Savar, Dhaka, Bangladesh

Key words: Jute Corchorus olitorius, Regeneration, Antioxidant, Vitamin, Senescence control

Abastract

Like nordihydroguaiaretic acid (NDGA), the addition of vitamin C and vitamin E (3 mg/1) in the rooting medium could control, through less effectively senescence of regenerated shoots of Corchorus olitorius. In the regenerating medium the addition of NDGA and vitamin C in combination was found to be highly effective in controlling senescence of the regenerating shoots and in obtaining maximum number of transferable shoots (9 shoots/explant). When the regenerating medium was supplemented with NDGA, vitamin C and vitamin E alone or NDGA plus vitamin E in combination could also control senescence but was less effective. The regenerated shoots grew normally when transferred to the antioxidant-omitted rooting medium.

Download Full Article