Plant Tissue Cult. & Biotech. 18(1): 7-16, 2008 (June)

Agrobacterium-mediated Genetic Transformation of Two Varieties of Jute (Corchorus capsularis L.)

R. H. Sarker, G.M. Al-Amin, Fathi Hassan and M.I. Hoque

Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka -1000, Bangladesh

Key words: Jute, Transformation, GUS expression, PCR analysis

Abastract

Transformation experiments were carried out using different explants of two varieties of white jute (Corchorus capsularis L.), namely, CVL-1 and CVE-3 with Agrobacterium tumefaciens strain (LBA4404/pBI121) containing the GUS and nptII genes. Maximum transformation ability was obtained from petiole-attached cotyledons and mature embryo explants. Kanamycin at a concentration of 200 mg/l was found optimum for selection of transformed shoots developed from mature embryos. Histochemical assay revealed the stable expression of the GUS gene within the various tissues of transformed plantlets. Stable integration of GUS and nptII genes were confirmed by PCR analysis of genomic DNA isolated from these transformed shoots.

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