Plant Tissue Cult. & Biotech. 17(2): 209-216, 2007 (December)

In vitro Plant Regeneration in Mungbean (Vigna radiata (L.) Wilczek)

M.I. Hoque, Most. Mosfeqa Zahan and R.H. Sarker

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh. E-mail : imdadul57@yahoo.com/rhsarker2000@yahoo.co.uk

Key words: Regeneration protocol, mungbean, Vigna radiata

Abastract

An efficient regeneration protocol without the intervention of callus using cotyledon explants of three mungbean varieties namely, BARI mug-3, BARI mug-5 and BINA mug-5 was developed. Best response toward multiple shoot regeneration in BARI mug-5 and BINA mug-5 was observed on MS supplemented with 0.5 and 0.5 mg/l Kn. However, for BARI mug-3, MS supplemented with 0.5 mg/l BAP and 0.1 mg/l Kn was found to be most effective for multiple shoot regeneration. Half-strength of MS supplemented with 0.5 mg/l IBA was found to induce healthy roots from the excised shoots in all the three mungbean varieties. In vitro regenerated plantlets with well developed roots were successfully established in soil.

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