Plant Tissue Cult. & Biotech. 17(2): 149-159, 2007 (December)

Bacteriophage Lambda Genomic Library Construction of Oryza sativa L. var. Pokkali

Suhaila Rahman¹, Masa-aki Ohto, Zeba I Seraj*¹ and Eduardo Blumwald

Department of Plant Sciences, University of California, Davis, CA, USA

Key words: Rice, Pokkali, Genomic library, Lambda vector, Partial Fill-In, Titer

Abastract

A major QTL called Saltol on rice chromosome 1 has been identified as linked to salinity tolerance traits of the landrace, Pokkali. A genomic library of Pokkali rice variety was constructed in bacteriophage Lamda Fix II with 20 kb inserts. Plaques containing genes implicated to salt tolerance have been identified using probes homologous to S-Ad methionine synthetase (SAM) and cation chloride co-transporter after two rounds of hybridization. Clearly demarcated plaques of the two clones of interest h

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