Plant Tissue Cult. 4(2), 105-109, 1994

In vitro Regeneration of Plants from Cotyledons of Momordica charantia L.

R. Islam, K.P. Sarkar, A.T.M. Naderuzzaman and O.I. Joarder

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Callus, Organogenesis, Momordica charantia, Tissue culture

Abastract

Cotyledon explants of Momordica charantia produced callus and sudsequently adventitious shoots on MS medium containing high concentration of an auxin along with a low one of a cytokinin. NAA-BA combination was suitable for regeneration and growth of shoots. Cotyledon explants proximal to the embryonic axis had the highest regeneration potential. Regenerated shoots elongated on MS medium with 0.5 mg/1 each of kinetin and GA₃. Rooting was induced when shoots were transferred to 0.1 mg/1 NAA-supplemented half-strength MS medium.

Download Full Article