lant Tissue Cult. & Biotech. 17(1): 37-46, 2007 (June)

Somatic Embryogenesis Induction in Narcissus papyraceus cv. Shirazi

Saghi Anbari, Masoud Tohidfar1, Ramin Hosseini2

No. 63, 10th Floor, 21 Building Omid Shahrak, Ghanat Kosar, Tehran, Iran

Key words: Narcissus, Somatic embryogenesis, Regenerati

Abastract

Explants from bulb scales of Narcissus papyraceus cv. Shirazi cultured in MS and Nitsch media containing different concentrations of BAP, 2,4-D, GA3, Kn, IBA and NAA produced highest number of regenerated bulblets in Nitsch medium containing BAP (2.2 mg/l) and 1.1 mg/l 2,4-D. The highest number of direct somatic embryos were observed in MS containing 0.5 mg/l GA3, 1.6 mg/l BAP and 1.6 mg/l 2,4-D. Induced somatic embryos were multiplied by transferring them to hormone free MS.

Download Full Article