Plant Tissue Cult. & Biotech. 16(1): 63-76, 2006 (June)

Review paper

Three Different Methods of Expression Profile Analysis in Plants

Mari L. Salmi, Thomas J. Bushart and Greg B. Clark

Department of Molecular, Cell and Developmental Biology, Scholl of Biological Sciences, The University of Texas at Austin, Austin TX 78712, USA

Key words:

Abastract

In the last 15 years many advances have been made in techniques available to molecular biologists. In the field of gene expression in particular, several new methods have become common use for determining changes in gene expression over the course of plant development, changes induced by external stimulus, and differences in the various tissue types of a plant. Here we describe the theory, general methods, and some applications for three of these new techniques in the broad field of plant biology DNA microarray, quantitative real-time reverse transcription PCR, and serial analysis of gene expression.

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