Plant Tissue Cult. 4(2) : 89-92, 1994

Control of Shoot-tip Necrosis during in vitro Establishment of Shoots of Pistachio

S. Mederos¹, Trujillo², I. L?pez¹

¹Dpto. Biologia Vegetal, Universided de La Laguna, Tenerife. ²Instituto de Productos Naturales y Agrobiologia de Canarias, C.S.I.C, Tenerife, Islas Canarias, Espa?a

Key words: In vitro, Pistacia vera, Shoot tips, Necrosis

Abastract

The most satisfactory protocol for in vitro establishment of shoots of Pistacia vera was obtained in solidified MS medium supplemented with 5mM NH₄NO₃ plus 0.4 mg 1^-1 thiamine-HCL, 0.4 1^-1 pyridoxine-HC1, 100 mg1^-1 myoinositol, 30g 1^-1 sucrose, 10^-6M BAP combined with 5.1^-8 M NAA. In this culture medium, shoots were more vigorous free from shoot-tip necrosis as observed in previously recommended 1/2 MS medium.

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