Plant Tissue Cult. & Biotech. 16(1): 45-52, 2006 (June)

Increased Regeneration Efficiency in Seed Derived Callus of Rice (Oryza sativa L.)

A. K. M. Mohiuddin, Shahanaz Sultana, Jannatul Ferdous

Biotechnology Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh

Key words: Rice varieties, Regeneration efficiency, Shoot regeneration,

Abastract

In five cultivated rice varieties e.g., Nonabokra, PNR 381, Taipei 309, Koshika and TKM 11 the response in the initiation of callus and its size varied greatly among varieties, salt concentrations (NaCl and Na₂SO₄) and their interaction. Better regeneration ability of Nonabokra, Koshika and TKM 11 was found to be correlated to small size callus with a high concentration of Na₂SO₄. The regeneration ability found here was dependent on variety/genotype, callus type and size, source of salt and their concentration. Regeneration ability enhanced three - tenfolds from Na₂SO₄ stressed callus compared to the control i.e. without Na₂SO₄. Vigorous rooting was also observed in the regenerated plants obtained from the calli induced in the medium containing Na₂SO₄. In NaCl supplemented medium, on the other hand, regeneration and rooting ability were poor.

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