Plant Tissue Cult. & Biotech. 16(1): 5-9, 2006 (June)

In vitro Propagation of Thuja occidentalis Through Apical Shoot Culture

M. H. Kabir, P. K. Roy and Golam Ahmed

Plant Biotechnology and Genetic Engineering Division, IFRB, Atomic Energy Research Establishment, Ganakbari, Savar, G.P.O. Box No. 3787, Dhaka-1000, Bangladesh

Key words: In vitro, Propagation, Thuja occidentalis, Apical shoot

Abastract

In vitro plant regeneration of Thuja occidentalis was obtained in apical shoot cultures from field grown plants. Hormone free MS medium 100 % explants produced shoots. The average number of shoots per explant was 6.57 ? 0.45 and the average shoot length of 4.5 ? 0.27 cm were recorded in this medium. Shoots rooted well when they were transferred into half strength MS with 1.0 mg/l IBA. The average number of root per shoot was 3.92 ? 0.28 and the average root length of 3.64 ? 0.38 cm were observed in this medium. No morphological variants were observed during the passage of in vitro culure.

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