Plant Tissue Cult. 2(2) : 97-101, 1992

Effect of BAP-Casein Hydrolysate on in vitro Multiplication of Leucana leucocephala from Different Explants

T.Hossain¹, M.Hossain, Shyamal K. Roy¹ and S.N Hossain

Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, P.O. Box 3787, Dhaka, Bangladesh

Key words: BAP-Casein hydrolysate, Multiplication, Leucaena

Abastract

Different explants, namely, cotyledonary node, stem node and shoot apex of Leucaena leucocephala were cultured on MS medium supplemented with 2.5 mg/1 BAP and 50-200 mg/1 casein htdrolysate. All explants produced multiple shoots. Maximum number of multiple shoot production was obtained from cotyledonary node explants on MS supplemented with 2.5 mg/1 BAP and 150-mg/1-casein hydrolysate. 0.5 mg/1 IBA-supplemented medium proved best for root formation. About 30% plantlets were successfully transferred into soil.

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