Plant Tissue Cult. 2(1): 31-34, 1992

MORPHOGENIC RESPONSES IN EXPLANTS OF LEUCAENA LEUCOCEPHALA (LAM.) DE WIT FOLLOWING DIFFERENT PHYTOHOMONES

M.Hossain, T. Hossain¹, Shyamal K. Roy¹ and S.N. Hossain

Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, P.O. Box. 3787, Dhaka, Bangladesh

Key words:

Abastract

Explants from cotyledon, hypocotyls and shoot apex were cultured on MS medium supplemented with BAP (0.2-3.5 mg/1) singly or in combinations with BAP (0.2-3.0 mg/1) and IAA, IBA and NAA (0.1-0.5 mg/1) at 26 ? 1 C under 16 h light period. Explants from cotyledon, hypocotyls and shoot apex produced multiple shoots on MS medium supplemented with 2.5 mg/1 BAP. Callus induction was 60 and 70 per cent at the base of hypocotyls and cotyledon explants in 3.0 mg/1 BAP + 0.5 mg/1 IAA.

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