Plant Tissue Cult. 3(1) : 11-15, 1993

Physico-chemical Changes and Plant Regeneration in Anther-derived Calli of Rice by Abscisic Acid Treatment

Nilufer H. Karim and F. J. Zapata*

Bangladesh Rice Research Institute, Joydebpur, Gazipur, Bangladesh

Key words: Abscisic acid, Sugar accumulation, Proline, Regeneration

Abastract

Abscisic acid at concentrations of 10, 20 and 30 mg/1 were used to observe changes in moisture accumulation, total protein, proline and sugar content and plant regeneration on the anther-derived calli of rice var. Taipei 309. Increasing ABA concentration resulted in decreased moisture accumulation. The total protein content did not show any marked variation. No proline accumulated due to increasing ABA levels. The highest accumulation of total sugar was observed at 20 mg ABA/1 with concurrent regeneration of green plants.

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