Plant Tissue Cult. & Biotech. 15(2): 121-126, 2005 (December)

Micropropagation of Aloe barbadensis Mill. Through In vitro Culture of Shoot tip Explants

R. Baksha, Miskat Ara Akhter Jahan, Rahima Khatun and John Liton Munshi

Biological Research Division, Dhaka Laboratories, BCSIR, Dhaka-1205, Bangladesh

Key words: Aloe barbadensis, Medicinal plant, Shoot tip, Micropropagation

Abastract

Multiple shoots (ten per explant) in Aloe barbadensis were obtained from shoot tip explant cultured on MS supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/1). About 95% rooting was obtained from micro-shoots cultured on half strength MS supplemented with NAA (0.5 mg/1). Well-developed rooted plantlets were successfully transferred to the soil with 70% survival.

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