Plant Tissue Cult. 15(1): 75-81, 2005 (June)

In vitro Regeneration and Multiple Shoot Induction in Upland Cotton (Gossypium hirsutum L.)

Saeed Rauf, Muhammad Usman*, B. Fatima and Iqrar A. Khan

Plant Tissue Culture Cell, Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan

Key words: Cotton, Cotyledonary nodes, Caulinar apex, Multiple shoots

Abastract

Cotyledonary nodes of Upland cotton (Gossypium hirsutum L.) produced maxi-mum number of shoots (3.43 shoots/explant) when cultured on MS supple-mented with 0.25 mg/l Kn. Highest percentage (93.3) of root development and root length (5.85 cm) was obtained when shoots were cultured on MS supplemented with 0.5 mg/l NAA and 0.1 mg/l Kn.

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