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Plant Tissue Cult. 15(1): 51-56, 2005 (June)

Callus Induction and Plant Regeneration from Rice Epicotyl

M. Mahmuda Khatun* and Nenita V. Desamero

Philippines Rice Research Institute, Maligaya, Science City of Munoz, Nueva Ecija, Philippines

Key words: Rice, Epicotyl, Callus formation, Plant regeneration

Abastract

Of the three rice genotypes, namely LX 297, IR 64 (1-1-4) and V 19 most responsive genotype LX 297 was found to be best for callus induction (30.93%) in MS medium. For regeneration, calli were transferred to MS-based regeneration medium MSK2. Highest regenerants (14.88%) were produced from calli induced on N6 medium when transferred to MSK2.

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