Plant tissue Cult. 4(1) : 53-57, 1994

In Vitro Plant Regeneration in Chrysanthemum morifolium Ramat

M.A. Khan, D. Khanam, K.A. Ara and A.K.M. Amzad Hossain

Horticulture Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Key words: In vitro regeneration, Chrysanthemum

Abastract

Plantlets were regenerated from shoot tips of mature Chrysanthemum morifolium cv. ?Super yellow? plants. Shoot proliferations were observed on MS medium supplemented with 0.5and 1.0 mg/1 BAP. At 2.0 mg/1 BAP shoot regeneration was higher but they were compact and stunted in growth. The shoots developed roots within 4-5 days in ? MS, ? MS + 0.25 mg/1 indole-3-butyric acid (IBA) and ? MS + charcoal. In ? MS with activated charcoal, root initiation was delayed and stunted in growth. All the regenerated plantlets were successfully transferred in pots in greenhouse and were well-established in soil.

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