Plant Tissue Culture 1 (1) : 51-56, June (1991)

REGENERATION OF SIX ORYZA SATIVA L. INDICA SALT TOLERANT VARIETIES FROM MATURE EMBRYOS

Z. I. Seraj, M. A. Samad, A. H. Talukder and M. A. Hossain

Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh

Key words:

Abastract

Regeneration capacity of callus cultures induced from mature seeds of six indica varieties was tested. The indica varieties were chosen due to their ability to grow in saline soils of regions of Noakhali (ECe3-12dS/m). The Aus or dry season varieties chosen were Chiknal, Binnatoa and Boilam and the Transplanted Aman varieties were Rajashail, Nona Balam and Patnai-23. By including BAP, Yeast Extract and decreasing the amount of 2, 4-D in the MS induction medium, it was possible to increase regeneration up to 37-41% in Nona Balam, 30-35% in Rajashail and 11-13% in Patai-23. Addition of tyrosine and IAA in the medium induction of calli of the Aus varieties, resulted in 30-33% regeneration in Binnatoa, 24-28% in Boilam and 22-25% in Chiknal.

Download Full Article