Plant Tissue Cult. 14(2) : 155-160, 2004 (December)

Studies on Callus Induction and Regeneration from Dehusked Rice (Oryza sativa L.) Seeds

M. M. Islam, S. A. Wahed and S. A. K. U. Khan

Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh

Key words: Dehusked rice, Induction, Callus, Regeneration

Abastract

The effect of varieties and media compositions on callus induction from dehusked rice seeds and subsequent plant regeneration was studied in the present investigation. Three rice varieties viz. Ranisalute, Chiniatap and Samudra Fena were studied. Among them, callus induction frequency was higher in Samudra Fena (53.33%). MS medium was used as basal medium supplemented with yeast extract (3 g/l), phenyl acetic acid (PAA) 1 mg/l, 2,4-D 1 mg/l and calcium silicate 66.66 mg/l. The medium supplemented with 2,4-D + calcium silicate showed better performance in cal1us induction (53.33%). Cal1i obtained from the same medium also showed maximum plant regeneration in all the three varieties. The variety Ranisalute performed better regarding plant regeneration (25%) in comparison with others.

Download Full Article