Plant Tissue Cult. 14(2) : 101-106, 2004 (December)

In vitro Multiple Shoot Regeneration from Nodal Explants of Zehneria scarba (L.F.) Sonder - An Important Medicinal Climber

S. P. Anand and R. Jeyachandran

Department of Plant Biology and Plant Biotechnology, St. Joseph?s College (Autonomous), Tiruchirappalli-620 002, Tamilnadu, India E.mail: jeyachandran02@yahoo.com/anand_srs@yahoo.co.in

Key words: Zehneria scabra, Nodal explants, Multiple shoot

Abastract

A protocol was developed for in vitro propagation by multiple shoot induction of Zehneria scabra (L.f.) Sonder, a medicinal climber having high medicinal values, belonging to Cucurbitaceae family. High frequencies of multiple shoot regeneration were achieved from nodal explants on MS fortified with 5 mg/l BAP and 0.5 mg/l IAA. Eight to ten shoots per explant were obtained. The elongated shoots were subcultured for rooting on MS supplemented with 2 mg/l NAA. The in vitro raised plantlets were acclimatized in green house and successfully transplanted to natural condition with 70% survival.

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