Plant Tissue Cult. 14(1) : 63-68, 2004 (June)

A Rapid Method for High Quality RNA Isolation from Jute : Corchorus capsularis L. and Corchorus olitorius L.

Farhan Khan, Ahmad Islam and Kanagasabapathi Sathasivan

Department of Molecular, Cell and Developmental Biology, School of Biological Sciences, The University of Texas at Austin, Austin, Texas 78712, U.S.A.

Key words: Jute, Corchorus capsularis, C. olitorius, RNA isolation, Polysaccharides, RT-PCR

Abastract

The isolation of RNA from herbaceous plants is difficult when there is an abundance of polysaccharides in the plant material. Corchorus capsularis and C. olitorius are two jute species, where conventional isolation procedures gave poor results. The present paper describes a modified method, which yields a greater quantity of RNA compared to the use of conventional protocols in both jute species. This procedure yielded 500 - 600 ?g of RNA per gram of fresh tissue and took only 3 h to complete. The RNA obtained was of high quality and proved suitable to Reverse Transcription-PCR.

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