Plant Tissue Culture 1 (1) : 35-41, June (1991)

CALLUS INDUCTION AND PLANT REGENERATION FROM DIFFERENT EXPLANTS OF ARACHIS HYPOGAEA L.

M. Imdadul Hoque, Shirin Mahbub, H.H. Sarker, M.M. Haque and A.S. Islam

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words:

Abastract

Callus culture of three local varieties, namely DA-1. DG-2 and Acc. No. 12 of Arachis hypogaea L. was established from different explants viz epicotyl, hypocotyl and cotyledonary nodal segments in MS medium supplemented with 2.0 mg/1 2, 4-D + 0.5 mg/1 kinetin. Shoot differentiated from the callus of epicotyl and hypocotyl explants when MS medium was supplemented with 1.o mg/1 each of BAP and NAA. A good number of multiple shoots regenerated without the intervention of callus from the cotyledonary nodal segment, cotyledon and shoot tip explants in MS medium containing 1.0 mg/1 BAP + 1.0/2.0 mg/1 IAA. Regenerated shoots developed root when they were transferred to half the strength of the MS medium with 0.2 mg/1 IBA.

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