Plant Tissue Cult. 14(1) : 37-43, 2004 (June)

Effect of Concentration of Sucrose on the Frequency of Callus Induction and Plant Regeneration in Anther Culture of Rice (Oryza sativa L.)

Sharmin Shahnewaz and M. A. Bari

Biotechnology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Induction, Callus, Sucrose, Albino plants

Abastract

Effect of various concentrations of sucrose in culture media on the frequency of callus induction and regeneration of green plantlets from anther culture of rice were investigated. Cold pretreated anthers of BRRI Dhan-29 at 6_C for seven days were cultured on N6 medium containing sucrose at concentrations of 0, 1, 2, 3, 4, 5, 6%; 2.0 mg/l 2,4-D; 0.5 mg/l Kn and 2.5 mg/l NAA. Results revealed that 4% sucrose was suitable for inducing high frequency (7.5%) callus induction and high green plant regeneration (65%). Highest concentration of sucrose (6% and above) in the culture medium not only resulted in an increase in the percentage of callus formation but also prompted the regeneration of albino plants.

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