Plant Tissue Cult. 13(2) : 155-163, 2003 (December)

In vitro Regeneration in Lentil (Lens culinaris Medik.)

R. H. Sarker, Barkat Murtaja Mustafa, Ashapurno Biswas, Shirin Mahbub, Mouful Nahar, Rehana Hashem and M. I. Hoque

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: In vitro, Regeneration, Lentil, Lens culinaris

Abastract

Four varieties of lentil (Lens culinaris Medik.), namely BM-1, BM-2, BM-3 and BM-4 of microsperma type were used in in vitro regeneration. Among various explants cotyledonary nodes from BM-2 and BM-4 were found to be the best for multiple shoot formation; decapitated embryos being the second best. Best multiple shoot regeneration was achieved on MS medium supplemented with 0.5 mg/l BAP, 0.5 mg/l Kn, 0.1 mg/GA3 and 5.5 mg/l tyrosine. Moderate success in root development was achieved on MS supplemented with 25 mg/l IBA.

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