Plant Tissue Cult. 13(2) : 145-150, 2003 (December)

Effects of Different Hormone Combinations and Planting Beds on Growth of Single Nodes and Plantlets Resulted from Potato Meristem Culture

S. A. M. Modarres Sanavy and M. Jami Moeini

Agronomy Department, Faculty of Agriculture, Tarbiat Modarres University, P. O. Box 14155-4838, Tehran, Iran (E-mail: Modaresa@Modares.Ac.Ir)

Key words: Potato, Micropropagation, Meristem culture, Single node cutting

Abastract

For this study, after thermotherapy, meristems of Agria and Marfona cultivars of potato (Solanum tuberosum L.) were placed on filter paper bridge in liquid medium. The meristem-derived plantlets were micropropagated in solid medium by using single node cuttings. Results showed that there are significant differences between Agria and Marfona cultivars. The modified solid (MS) medium without NAA and BAP was found to be best for the formation of roots and shoots. Addition of BAP and NAA in the medium decreased shooting and rooting, respectively of single node cultures. A mixture of peat moss and sand in the ratio of 4 : 1 proved best for growing plantlets.

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