Plant Tissue Cult. 13(2) : 99-107, 2003 (December)

Effect of Genotype and Culture Media on Callus Formation and Plant Regeneration from Mature Seed Scutella Culture in Rice

M. Mahmuda Khatun¹, M. Hazrat Ali² and Nenita V. Desamero

Philippines Rice Research Institute, Maligaya, Science City of Munoz, Nueva Ecija, Philippines

Key words: Rice, Scutella, Callus formation, Plant regeneration

Abastract

In vitro plant regeneration from callus induced from mature seed scutella of four rice genotypes, Lx297, IR64, V19 and IR64-1-1-4 was studied. Three basal media, namely N6, MS and R were tested for callus induction; of these MS was found best for callus induction. The genotype Lx297 was found most responsive and yielded highest percentage (82.50) of callus on MS. Results also showed that callus induced on R medium gave the highest regeneration frequency (13.89%) when transferred to MSK₂ regeneration medium.

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