Plant Tissue Cult. 13(1) : 61-73, 2003 (June)

Epigenesis and High Frequency Plant Regeneration from Soybean (Glycine max (L.) Merr.) Hypocotyls

Manoj Tripathi and Sharad Tiwari

Plant Biotechnology Laboratory, Department of Plant Breeding and Genetics, Jawaharlal Nehru Agricultural University, Jabalpur 482004 MP, India

Key words: Soybean, Glycine max, Hypocotyl, Epigenesis, Plant regeneration

Abastract

High frequency somatic embryo induction was achieved in Glycine hypocotyls depending upon genotype and the explant inoculation medium. Plant regeneration efficiency was in excess of 85% following plural shoot initiation in genotype JS 90-41 when the in vitro inoculation background was a combination of 0.5 mg/l NAA and 3.0 mg/l BAP and contained MS macro- and micro-nutrients, vitamins and agar. Phenotypically normal plants were regenerated from the hypocotyl explants.

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