Plant Tissue Cult. 12(2) : 173-180, 2002 (December)

Ogganogenesis in Teasle Grourd (Momordica dioica Roxb.)

S. A. Nabi¹, M. M. Rashid², M. Al-Amin³ and M. G. Rasul

Department of Genetics & Plant Breeding, Bangabandhu Sheikh Mujibur Rahaman Agricultural University, Salna, Gazipur-1703, Bangladesh

Key words: Organogenesis, Micropropagation, Momordia dioica

Abastract

Of the four types of explants of teasle ground (Momordica dioica Roxb.) viz. node, shoot tip, leaf and the cotyledon, the cotyledon showed the best performance. The combination 1.0 mg/1 BAP and 0.1 mg/1 NAA was found most suitable in callus induction followed by 0.2 mg/1 NAA. The highest number of multiple and tallest shoots were obtained on MS medium fortified with 1.0 mg/1 BAP and 0.1 mg/1 NAA. For rooting, half strength MS supplemented with IBA proved to be better than IAA, although on half strength MS supplemented with IAA tallest shoots were observed.

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