Plant Tissue Cult. 12(2) : 93-97, 2002 (December)

Plant Regeneration from Node and Internode Explants of Solanum trilobatum L.

D. I. Arockiasamy, B. Muthukumar¹, E. Natarajan and S. John Britto

Centre for Natural Resources Studies, Department of Botany, St. Joseph?s College, Tiruchirappalli-620002, India

Key words: Solanum trilobatum, Micropropagation, Node, Internode

Abastract

Shootlets were regenerated from node and explants of Solanum trilobatum L. through direct and indirect organogenesis. The induction of multiple shoots from nodal explants was high in LS medium supplemented with a combination of 5 mg/1 BAP and 0.05 mg/1 IAA. Shootlet regeneration from internodal explants was also high in LS medium with a supplement of 5 mg/1 BAP and 0.05 mg/1 NAA. The regenerated shootlets were rooted on LS medium fortified with different concentrations of IBA. The maximum percentage of rooting was obtained with 1 mg/1 IBA. The rooted plantlets were hardened and successfully established in soil.

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