Plant Tissue Cult. 12(1) : 1-9, 2002 (June)

In vitro Synthesis of White Grained Primary Hexaploid Triticales

Jaswinder Kaur, C. K. Satija and S. S. Gosal

Department of Genetics and Biotechnology, Punjab Agricultural University, Ludhiana-141 001, India

Key words: Tricitum durum, Embryo culture, White rye, Amphidiploidy

Abastract

White grained primary hexaploid triticales were synthesized by culturing hybrid embryos of Triticum durum with white rye as well as from callus derived from hybrid embryos on artificial nutrient medium. Fully developed embryos resulted by applying 2,4-D after pollination for six - seven consecutive days. The emblings regenerated from embryos and callus were studied mitotically showing the 2n = 3x = 21 chromosome, confirms the hybrids. The amphidiploidy was induced by injecting 0.25% aqueous colchicine at booting stage using hypodermal needle. Colchiploid plants were studied meiotically showing 2n = 6x = 42 chromosome. Pollen fertility ranged from 40.07 - 55.68 % in amphidiploids.

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