Plant Tissue Cult. 3(2) : 107-110, 1993

High Frequency Plant Regeneration from Cotyledon Cultures of Aegle marmelos Corr.

R. Islam, M.R. Karim, A. Haque, M. Hossain and O.I. Joarder

Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Key words: Callus, Cotyledon, Aegle marmelos, Tissue culture

Abastract

Cotyledons from seeds of mature but green unripe fruits of Aegle marmelos were used for inducing callus. Optimum callus formation was observed on MS medium supplemented with 2 mg/1 2,4-D and 1 mg/1 benzyladenine (BA). Maximum regeneration occurred upon transferring calli to the medium containing 1 mg/1 BA and 0.1 mg/1 IAA. Regeneration shoots rooted readily (70%) on solidified MS medium containing 30 mg/1 IBA.

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