Plant Tissue Cult. 3(1) : 5-9, 1993

Direct Enbryogenesis from Bulb-scale Explant of Eucharis grandiflora

A. Mujib, S. Bandyopadhyay, B. K. Jana* and P. D. Ghosh

Birla Laboratory, The Agri-Horticultural Society of India, 1, Alipore Road, Calcutta-700 027, India

Key words: Callus, Regeneration, Embryogenesis, Eucharis

Abastract

Bulb-scales excised from bulb product somatic embryos directly without callus formation when cultured on MS basal medium supplemented with either NAA/BAP or BAP alone. The somatic embryos resembling water droplets, were transparent at the initial stage became opaque and developed into plantlets after passing a long dormant phase. The significance of development of non-zygotic embryos vis-?-vis in vitro cloning is discussed.

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