Plant Tissue Cult. 11(2) : 173-179, 2001 (December)

In vitro Regeneration of Plantlets from Shoot tip and Nodal Segments in Nayantara (Catharanthus roseus L.)

M. R. Islam, S. N. Hossain¹, M. K. Munshi, L. Hakim and M. Hossain

Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), G.P.O. Box 3787, Dhaka-1000, Bangladesh

Key words: Catharanthus roseus, Shoot tip, Nodal segment, Regeneration

Abastract

Best response towards multiple shoot regeneration was obtained from the nodal segments of Catharanthus roseus on MS supplemented with 1.5 mg/l BAP + 0.2 mg/l NAA. In this combination nodal segments produced 20 - 24 initial shoot buds whereas shoot tip produced only 12 - 16 shoot buds. Regenerated multiple shoots did not produce roots. Among the three auxins (IAA, NAA and IBA) tried for root induction, half strength MS containing 0.2 mg/l IBA was found to be effective in the production of roots. About 90% of the regenerated plantlets survived in the field condition.

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