Plant Tissue Cult. 11(2) : 121-126, 2001 (December)

In vitro Multiplication of Gladiolus (Gladiolus crassifolius)

Iqbal Hussain, Aish Muhammad, Hamid Rashid and Azra Quraishi

Agricultural Biotechnology Institute, NARC, Park Road, Islamabad, Pakistan.
E-mail: abi_narc@yahoo.com

Key words: In vitro, Multiplication, Gladiolus crassifolius, Shoot tip

Abastract

In vitro multiplication of gladiolus with exogenous growth regulators was studied by using shoot tips. Buds (1 - 2 mm) excised from the field grown bulbs were cultured on MS solid and liquid medium supplemented with BAP and Kn at 0.0 - 4.0 mg/l each along with 40 g/l sucrose. The response for bud initiation was better on solid medium while liquid media proved better for maintenance and multiplication. Multiplication rate was higher when MS medium was supplemented with BA 3.0 and 4.0 mg/l for Pink and White variety, respectively.

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