Plant Tissue Cult. 11(1) : 41-45, 2001(June)

Regeneration of Plantlets from Cotyledon Explants of Lablab bean (Lablab purpureus (L.) Prain)

Muthu Thiruvengadam and Narayanasamypillai Jayabalan

Plant Tissue Culture Laboratory, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India.
E-mail: thiruv@bdu.ernet.in

Key words: Lablab purpureus, Regeneration, Cotyledon explant

Abastract

High frequency callus induction and shoot multiplication in lablab bean (=Lablab purpureus var. lignosus) was observed in MS medium containing NAA, BAP and CW. The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0.5 mg/1) along with 10% (v/v) coconut water. Maximum percentage of shoot bud formation and multiplication was obtained from BAP (1.0 mg/1) and NAA (0.1 mg/1). The well developed shoots were transferred to MS medium containing IBA (1.5 mg/1) for induction of roots. The rooted plants were transferred to plastic cups and finally to field.

Download Full Article