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Plant Tissue Cult. 3(2) : 97-100, 1993

Regeneration through Internodal Callus of 40-Year -Old Melia azadarch

Zaheer Ahmad, Nasrren Zaidi and F. H. Shah

Dr. Bashir Ahmed Reasearch Centre, PCSIR Laboratories Complex, Ferozepur Road, Lahore-54600, Pakistan

Key words: Regeneration, International Callus, Melia azadarch

Abastract

Shoot regeneration in calluses of Melia azaddarch was achieved on MS medium containing 0.5-1.0 mg/1 of BAP. Regenerated shoots were rooted in half strength MS medium supplemented with 1 mg/1 IBA. Contamination rate of cultures due to seasonal changes has also been reported.

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