Plant Tissue Cult. 10(1) : 89-92, 2000 (June)

Short Communications

High Frequency Plantlet Regeneration Through Callus for Improvement in Sugarcane Variety CoSe 95436

J. P. Tripathi¹, S. K. Singh and S. B. Singh

G. S. Sugarcane Breeding and Research Institute, Seorahi, P. O. Tamkuhi Raj, Dist. Kushinagar-274407, U. P., India

Key words: Plantlet regeneration, Callus culture, Sugarcane

Abastract

Callusing of sugarcane variety CoSe 95436 was induced on MS Medium supplemented with 5.0 mg/1 2,4-D from apical leaf rool and apical shoot meristems. Shoot organogenesis was tested at different Kn and BAP leves added as supplement to MS medium. In vitro shoot multiplication and growth were optimal at 3.0 mg/1 Kn. At this concentration of Kn, there was 8.7-fold increase of shoot multiplication in 30 days culture, while the increase was 6.7-fold at MS supplemented with 1.5 mg/1 BAP.

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