Plant Tissue Cult. 10(1) : 39-44, 2000 (June)

In vitro Propagation of Datura metel L. from Hypocotyl Explants

B. Muthukumar, D. I. Arockiasamy and S. John Britto

Centre for National Resources Studies, St. Joseph?s College (Autonomous), Tiruchirappalli-620 002, Tamil Nadu, India

Key words: Datura metel, Hypocotyl, In vitro, Propagation

Abastract

Plants were efficiently regenerated from hypocotyls explants of Datura metel L. on MS basal medium with different concentrations of BAP and 2,4-D. Maximum percentage of shoot proliferation was observed at 1.5 mg/1 BAP followed by 1.0 mg/1 BAP. The regenerated shoots were elongated on MS basal medium without any growth regulator and rooted with different concentrations of IBA. The percentage of rooting was maximum at 2.0 mg/1 IBA. The rooted plantlets were hardened properly and transferred to soil.

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