Plant Tissue Cult. 3(2) : 85-89, 1993

Direct and Indirect Organogenesis in Cultured Hypocotyl Explants of Abelmoschus esculentus (L.) Moench.

S.A. Haider, R. Islam, A.H.M. Kamal, S.M. Rahman and O.I. Joarder

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Abelmoschus esculentus, Organogenesis, Hypocotyl explants, Plant regeneration

Abastract

In Abelmoschus esculentus plant regeneration was achieved from hypocotyls explants via direct and indirect organogenesis depending on the ratio of cytokinin and auxin. MS medium when supplemented with 1-3 mg/1 benzyladenin (BA) and 0.1-0.3 mg/1 a-naphthaleneacetic acid (NAA) induced direct shoot organogenesis whereas the medium containing 0.1-0.3 mg/1 BA and 1-3 mg/1 NAA supported callus growth without shoot formation. The callus when subcultured on to the medium containing 1 mg/1 BA and without auxin shoot regeneration was achieved. Complete plantlets were obtained upon transfer of the regenerated shoots on the MS medium with 0.1 mg/1 indole-3-butyric acid (IBA).

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