Plant Tissue Cult. 9(2) : 167-172, 1999 (December)

Regeneration of Plantlets from the Callus of Stem Explants of Hybanthus enneaspermus (L.) F. Muell.

E. Natarajan, D. I. Arockiasamy and S. John Britto

Centre for Natural Resources Studies, St. Joseph?s College (Autonomus), Tiruchirapalli, Tamilnadu-620 002, India

Key words: Hybanthus enneaspermus, Callus, Regeneration, Plantlets

Abastract

Callus-mediated shoot regeneration from stem explants of Hybanthus enneaspermus is described. Green compact nodular calli are obtained on MS medium containing 2.0 mg/1 2,4-D and 0.5 mg/1 BAP. Shoot buds were induced and elongated on MS basal medium with 5.0 mg/1 BAP. The shoots were rooted in MS medium with 2.0 mg/1 IBA, and then were successfully hardened and transferred to the field.

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