Plant Tissue Cult. 3(2) : 59-65, 1993

High Transient Gene Expression, with Conserved Viability, in Electroporated Protoplasts of Glycine, Medicago and Stylosanthes Species

B. Jones, G. Antonova-Kosturkova, M.L.C. Vieira, E.L. Rech, J.B. Power and M.R. Davey

Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, University park, Nottigham NG7 2RD, U.K.

Key words: Trans gene expression, Conserved viability, Electroporated protoplasts, Glycine, Medicago, Stylosanthes

Abastract

A non-commercial, direct current square wave generator was used to induce the uptake of plasmid into plant protoplasts. High levels of transient gene expression were obtained without severe loss of protoplast viability in the leguminous species Glycine argyrea, Medicago sativa, M. varia and Stylosanthes macrocephala.

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