Plant Tissue Cult. 8(1) : 149-156, 1998 (December)

Cytological Studies in Brassica spp. From Cultured Cells Supplemented with 2, 4-D

Neelu Sood

Department of Biochemistry, Guru Nanak Dev University, Amritsar-143 005, India

Key words: Cytological studies, Brassica spp.., Polypoidy, Somatic meiosis

Abastract

Cytological studies of the calli raised from various explants viz. stem, cotyledone, hypocotyl, root and floral bud of Brassica camprestris var. Yellow sarson (2n = 20), B. juncea var. RLM 619 (2n=36) and B. alba (2n=24) were carried out. Mixoploidy was observed in callus cultures and diploid cells were most common besides triploid, tetraploid and pentaploid. Polyploidy was more common in B. campestris and B. juncea. No cytochimerism was observed in B. alba cultures. The meiotic like behaviour was observed in somatic cell cultures of B. juncea in floral bud callus.

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