Plant Tissue Cult. 8(1) : 61-68, 1998 (June)

Studies on In vitro Propagation of Carnation (Diathus caryophyllus L.)

Manreet Sooch, J. S. Arora, Kushal Singh and S. S. Gosal¹

Department of Floriculture and Landscaping, Punjab Agricultural University, Ludhiana-141 004, India

Key words: Carnation, In vitro propagation, Multiple shoots

Abastract

MS medium supplemented with BAP (0.5 mg/1) was found optimum for shoot proliferation in carnation cv. Scania. Addition of NAA or BAP supplemented medium enhanced shoot elongation. High BAP level caused vitrification of shoots. Vitrified shoots reverted to normal when transferred to hormone free MS medium. Half strength MS medium containing IBA (0.5 mg/1) was found most suitable for rooting of in vitro raised shoots. Plants showed very high percentage of survival when hardened under high relative humidity for three weeks.

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