Plant Tissue Cult. 8(1) : 55-60, 1998 (June)

Effect of Thidiazuron and BAP on Shoot Buds Differentiation from Excised Cotyledons of Brassica juncea (L.) Czern.

Nidhi Prabhakar

Department of Botany, University of Delhi, Delhi-110 007, India.

Key words: Brassica juncea, Regeneration, Thidiazuron.

Abastract

A comparative study of the effect of BAP and thidiazuron (TDZ) on shoot bud differentiation from excised cotyledons of Brassica junea cv. PR-45 showed that the latter is ten times more effective than the former and in combination produces synergistic effect. At its optimum concentration (5 ? 10^-4 mM) TDZ induced 100% regeneration with 7.4 shoots per cotyledon.

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