Plant Tissue Cult. 8(1) : 11-18, 1998 (June)

Transient GUS Expression in Jute (Corchorus capsularis L. Var. D-154) Explants after Infection by Agrobacterium tumefaciens

A. B. M. Mamun Hossain, Golam Ahmed, R. I. Khan¹ and M. Serajul Islam

Division of Genetics, Institute of Food and Radiation Biology, AERE, Savar, Dhaka, Bangladesh

Key words: GUS expression, Corchorus capsularis, Agrobacterium tumefacien

Abastract

Two gene contructs, BSF 16 (carrying GUS gene and Basta resistant gene) and Tab7 (carrying GUS gene and kanamycin resistant gene) were used for insertion of useful genes into jute tissues through Agrobacterium tumefaciens infection. Hypocotyl, plumules and cotyleonary bases with petiole from 24 and 48 h old germinating seedlings were used as explants. Successful delivery of genes took place in all types of explants as was evident by GUS assay. The rate of delivery of genes was highest in 48 h old plumule explants of followed by cotyledonary base explants. The gene was delivered into the cut surface of the meristematic zone of both plumules and cotyledonary bases from where multiple shoot regeneration took place. However, no transgenic plants grew to maturity.

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