Plant Tissue Cult. 8(1) : 1-10, 1998 (June)

Micropropagation through Seeds and Shoot Segments in Dendrobium crepidatum Lindl.

P. Das Gupta, M. Al-Forkan and S.K. Bhadra

Department of Botany, University of Chittagong, Chittagong-4331, Bangladesh

Key words: Dendrobium crepidatum, Micropropagation, Media effect

Abastract

Seeds of Dendrobium crepidatum were especially grown on four different 0.8% (w/v) agar solidified basal media, namely PM, MVW, MS and MK. Seeds germinated on all these media but they took less time in the former two than in the latter. In vitro germinated seedlings underwent rapid elongation in hormone supplemented liquid and 0.8% (w/v) agar solidified media. In terms of the increase in shoot system, liquid MS + 2% (w/v) sucrose + 1.0 mg/1 NAA + 3.0 mg/1 BAP + 1.5 mg/1 Kn was the best followed by liquid MVW + 2% (w/v) sucrose + 1.0 mg/1 NAA + 3.0 mg/1 BAP + 1.5 mg/1 Kn and the others. The seedlings developed poor root system on the elongation media. Thus in order to induce strong and stout root system, these were transferred onto 0.8% (w/v) agar solidified (i) half strength MS + 1.5% (w/v) sucrose and (ii) MS + 3% (w/v) sucrose + 0.5 mg/1 IAA. In terms of both number of roots developed and increase in root system, half strength MS was better. For further multiplication, the shoot segments from the in vitro germinated seedlings were cultured on 0.8% (w/v) agar solidified (i) MS + 3% (w/v) sucrose + 0.01 mg/1 IAA + 2.0 mg/1 BAP and (ii) MS + 3% (w/v) sucrose + 0.1 mg/1 IAA + 1.0 mg/1 BAP media. Multiple shoot buds developed from the nodal zone of he shoot explants in both the media and the former proved better than latter. The multiple shoot buds underwent elongation on the media but did not produce root system. The shoot buds of 2-3 cm long were individually cultured on two different 0.8% (w/v) agar solidified media, namely (i) half strength MS + 1.5% (w/v) sucrose and (ii) MS + 3% (w/v) sucrose + 0.5 mg/1 IAA. Some of these in vitro grown seedlings were successfully transferred outside where continued their growth.

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