Plant tissue Cult. 7(2) : 125-133, 1997 (December)

In vitro Propagation of Neem (Azadirachta indica A. Juss) Plants from seedlings explants

R. H. Sarker, M. Rafiqul Islam and M. I. Hoque

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Neem plants, Azadirachta indica, Micropropagation

Abastract

Among the different explants such as shoot tip, nodal-, leaf-and hypocotyl segments of in vitro grown neem plants (Azadirachta indica A. Juss), nodal segment was found to be best for multiple shoot regeneration on MS medium containing 2.0 mg/1 BAP and 0.2 mg/1 NAA. Shoot development was also tried through callus culture. For this the best callus response was obtained from internode, cotyledon and leaf segments when cultured on MS medium supplemented with 4.0 mg/1 BAP. A large number of shoot buds were produced when such four week-old-calluses were subcultured on the same medium containing 10.0 mg/1 BAP. The regenerated multiple shoots were devoid of any root. Roots were induced when the isolated individual shoots were cultured on half-strength MS medium containing 0.2 mg/1 IBA. More than 90% of the plantlets thus obtained were successfully established in the soil.

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