Plant Tissue Cult. 7(1) : 53-61, 1997 (June)

In vitro Propagation of Carissa carnandus L. Using Seedlings Explants

M. N. Amin*, R. Begum, M. A. Rahman and M. A. K. Azad

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: In vitro multiplication, Seedlings explants, Carissa carandus

Abastract

Seedlings raised in vitro on MS medium were used as explants for establishing multiple shoots of Carissa. Explants taken from the seedlings grown in vitro on medium supplemented with BA showed early sprouting and development of axillary shoots than those taken from the seedlings grown on cytokinin-omitted medium. Explant taken from middle node was found to be superior to cotyledonary node and shoot tip in proliferating axillary shots on MMS₁ containing 0.5-1.0 mg/1 BA. Nodal segments taken from the in vitro proliferated shoots were subcultured on the proliferation medium for maintaining shoots were at a multiplication rate of six to eight-fold per six weeks. About 93% in vitro proliferated shoots rooted in 0.5 mg/1 IBA containing MMS₂ medium. Continuous presence of the auxin in rooting medium induced basal callusing thus inhibiting emergence and healthy roots. Formation of roots without basal callusing could be achieved by transferring the microcuttings to the auxin-free medium after one week of auxin treatment or by adding 1.0 mg/1 activated charcoal to the medium containing auxin.

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