Plant tissue Cult. 7(1) : 13-19, 1997 (June)

Rapid Propagation of Dalbergia sissoo from Mature Trees Through Tissue Culture

S. S. Gill, R. I. S. Gill and S. S. Gosal¹

Department of Forestry and Natural Resources, Punjab Agricultural University, Ludhiana- 141 004, India

Key words: Dalbergia sissoo, Micropropagation, Asexual multiplication

Abastract

Shoot cultures were established from axillary buds excised from coppice shoots of mature Dalbergia sissoo trees. The nodal buds regenerated into shoots were maintained and multiplied through periodic subcul-turing on MS medium supplemented with different concentrations of cytokinins and auxins. The nodal segments exhibiting multiple shoot regeneration varied greatly with the position of donor branch. Among the explants tested for multiple shoot regeneration on MS with BAP (1.0 mg/1) and NAA (0.2 mg/1), the nodal segments taken from basal and middle portions responded better than those excised from apical portion. The elongated shoots were rooted successfully through culturing on half-strength MS medium supplemented with IBA (1.0 mg/1) and NAA (0.2 mg/1). The complete plantlets thus developed were successfully transferred to field.

Download Full Article