Plant Tissue Cult. & Biotech. 34(2): 115-121, 2024 (December)

General

Direct In vitro Regeneration of Sugarcane var. China (Saccharum officinarum L.) Using Leaf-Sheath Explants

M.H. Kabir, Pronabananda Das, Md. Monirul Islam and A.N.K. Mamun

Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, Post: DEPZ1349, Savar, Dhaka, Bangladesh

Key words: Direct plant regeneration, In vitro, Leaf sheath explants, Sugarcane, MS

Abastract

An efficient and reproducible in vitro regeneration protocol was developed for sugarcane var. China using leaf-sheath explants. Direct multiple shoot formation without the intervention of callus from leaf-sheath was achieved on MS medium supplemented with 10.0 mg/l NAA. Ninety percent of the explants were found to induce shoots following a 90-day culture period. After 90 days of cultivation, each explant produced an average of 30.0  2.50 shoots having a mean shoot length of 7.2  0.50 cm on this medium. Within 30 days of being exposed to 1/2 strength of MS containing 2.50 mg/l NAA, 90% of the regenerated shoots produced roots. The average root length in this medium was 7.50  2.30 cm, and there were 15.0  1.25 roots produced from each shoot. Ninety percent of the in vitro raised plantlets were found to survive in the soil under a natural environment

Download Full Article